Introduction: Median nerve is one of the terminal branches of the brachial plexus. It is formed by union of two roots, lateral root and medial root coming from lateral and medial cord respectively. Formation of median nerve occur lateral to axillary artery in axilla. Median nerve is associated with several variations which include abnormal communications with other nerves such as musculocutaneous and ulnar nerves, splitting of the nerve, penetration of the nerve by other vessels such as brachial artery. In the present study we observed anatomical variations of median nerve formation. Material and methods: We studied anatomical variations of median nerve formation in 98 cadavers i.e. 196 upper limbs of formalin preserved cadavers. Results: We observed following Anatomical variations of median nerve formations- Three roots taking part in the formation of median nerve in 22.4% upper limbs. Four roots taking part in formation of median nerve in 3.57% upper limbs. Median nerve formation in the arm was seen in 17.3% upper limbs, median nerve formation medial to axillary artery in 6.12% upper limbs and median nerve formation anterior to axillary artery in 1.53% upper limbs. Conclusion: Knowledge of such anatomical variations is of interest to the anatomist and clinician alike. Surgeons who perform procedures involving neoplasm or repairing trauma need to be aware of these variations.