Aim: Test the efficiency of a device and a specific protocol to stimulate muscular hypertrophy of an upper limb body muscle in rats. Method: We used 16 male Wistar rats divided into 3 groups: Initial Control (SC), Aged Control (SE), and Resistance training (T). We applied morphoquantitative measurements such as cross sectional area, myocites (Vv[myo]) and interstitial (Vv[inters]) volume density in the Triceps Brachilis muscle (TB). The Ladder climbing equipment was utilized and each rat performed a total of 6 climbs with a 45 seconds of rest between each climb, in a frequency of five times per week during 16 weeks. The imposed overload was established according to a table for humans proposed and adapted from Heyward. Results: The animals reached 200% of the initial training load at the end of the training period. Morphometric analysis showed a 45% increase in the cross sectional area of the T group compared to SE (p<0.05). The stereological analysis confirmed also an increase in the Vv[myo] of the group T in comparison to SE. The Vv[inters] of the groups SC and T presented a meaningful difference in comparison to SE (p<0.05). Conclusion: the resistance training device and protocol used was efficient to demonstrate significant morphoquantitative alteration in the TB muscle size. The present results affirm that Ladder climbing equipment can also be used to induce upper limb muscle hypertrophy.