Heteropterys aphrodisiaca infusion, alone or associated with endurance training, was investigated in rat bones in relation to their mechanical properties, collagen content and morphology. Male rats were divided into four groups (n = 8): CS- control sedentary, HS- H. aphrodisiaca sedentary, CT–control trained, HT‑H. aphrodisiaca trained. The training protocol consisted in running on a motorized treadmill, 5 times a week, for 8 weeks, with weekly increase in treadmill velocity and duration. Control groups received water while HS and HT groups received H. aphrodisiaca infusion (104 mg/animal) by gavage during the 8 weeks. Tibiae were frozen for collagen dosage and biomechanical analysis or preserved in Karnovsky’s fixative, then processed for histomorphological analysis by conventional light microscopy and scanning electron microscopy. The HT group showed significantly higher yield load and yield stress in the tibiae three-point bending test. The maximum load, stiffness, maximum stress and elastic modulus were statistically similar for the experimental groups. The hydroxyproline content, morphometrical and stereological data were not significantly different for the four groups. Scanning electron microscopy showed more lacunae and Havers canals in the bone of trained animals, moreover the osteons were more disorganized, when compared with sedentary groups. These alterations may indicate that the bone of trained animals was being remodeled. However, after 8 weeks of training, it was not possible verify alterations in morphometrical measurements, collagen content, stiffness and modulus of elasticity of the trained and treated animals.