There may be significant variations in the distance between the carotid sulci on both sides of the sphenoid bone. This distance varies throughout the entire length of the carotid sulci. It is important to be aware of the anatomical variations in these distances during trans-sphenoidal surgery to avoid potential catastrophic injury to the carotid arteries. The distance between the carotid sulci was measured in 26 disarticulated sphenoid bones at the anterior most and posterior most points on the medial borders of the carotid sulci and at the level of maximal sellar depth. The shortest distance was found to be at the anterior most and posterior most points in about 35% each of all specimens studied while in about 26% of specimens the shortest distance was at the level of maximum sellar depth. The minimum inter carotid distance was found to be 7.63 mm. The present study documented significant variations in the inter carotid sulci distances along the course of the carotid artery in the cavernous sinus. The knowledge of this fact may be of possible help while planning transsphenoidal surgery and in avoiding carotid artery injury.