The production of culture and capture fishery of Indian major carps and Exotic carps coming to a standstill in recent years, even with various technical and managerial inputs, the question over its biodiversity has been ask frequently. The traditional method of morphometric observation along with anatomical and biochemical analysis has been conducted in farme3d reared composite species of Indian major carps and Exotic carps to study its biodiversity. Morphometric measurement in eighteen external characters were analysed with ANOVA (P < 0.05) and found significance. Critical analysis of each character by pair-wise comparison gives significant among species in some of characters. Anatomical study with respect to their body length recorded in gill, heart, and kidney gives the general difference in physiological and habitat role played by this organs.