Some groups of bees collect oil from flowers and use this product to feed the larvae and to line the nests and brood cells, as is the case for bees of the Neotropical genus Tetrapedia (Tetrapediini, Apidae). They are solitary and construct their nests on pre-existing cavities in wood. Aiming to bring a better understanding of the oil collecting structures of Neotropical oil bees, in this study we examined the foreleg morphology of female of Tetrapedia diversipes Klug, showing on SEM the adaptations of forebasitarsus for collecting oil from flowers. The metasoma of female bees was measured and dissected using stereomicroscope and the size and shape of the Dufour’s gland were estimated. T. diversipes hold a curved comb on the basitarsus of the front leg to collect oil and a mixture of slender and branched hairs on the scopa of the hind leg to transport it. These structures are very similar on other examined Tetrapedia species. The Dufour’s gland of T. diversipes is reduced, occupying about 2.2% of the metasoma. Further investigation of the chemical composition of the Dufour’s gland secretion, of the cell lining and of the collected floral oil might clarify the role of these components on T. diversipes’ life.