Journal of Morphological Sciences
http://www.jms.periodikos.com.br/article/587cb4617f8c9d0d058b4638
Journal of Morphological Sciences
Original Article

HISTOCHEMISTRY AND GROWTH CHARACTERISTICS OF BOVINE SEMITENDINOSUS MUSCLE EXPOSED TO RECOMBINANT BOVINE SOMATOTROPIN (rbST)

Fernanda Regina Carani; Paulo Sérgio Andrade Moreira; Robson Francisco Carvalho; Carlos Roberto Padovani; Luiz Artur Loyola Chardulo; Maeli Dal Pai Silva

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Abstract

Bovine skeletal muscle growth characteristics and muscle fiber type frequency are of primary interest because they both play a fundamental role in modeling meat quality and tenderness, although the precise relationship remains undefined. Growth promoters like rbST have been reported to have varying effects on muscle growth performance. The objective of this experiment was to evaluate the histochemistry and growth characteristics of bovine semitendinosus muscle treated with rbST. Animals were divided into two groups: control (saline-injected; n=8) and rbST-injected (15 μg/kg; n=8). Heifers were injected every 14 days from day 210 until day 285 of age. Muscle samples were collected (day 210 and 360) and frozen in liquid nitrogen. Histological sections (10 μm) underwent morphological and histochemical analysis (HE, NADH-TR and mATPase), morphometry (fiber area and distribution), and biochemical analysis. Fibers were classified as SO, FOG, and FG. FOG fiber percentage distribution decreased and cross-sectional area increased in rbSTtreated animals. Recombinant bST caused a greater animal body weight gain and FOG fiber hypertrophy, while contributing to a decrease in FOG fiber distribution. We conclude that the phenotypic modulation seen in this muscle fiber suggests a potential role of this muscle in modeling the meat quality.

Keywords

Beef cattle, fiber types, histochemistry, muscle growth, rbST
587cb4617f8c9d0d058b4638 jms Articles
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J. Morphol. Sci.

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