In the present study, the myocardial bridges of an adult, female, mountain lion that died of natural causes at the “Parque do Sabiá” Zoo, Uberlândia, Minas Gerais, Brazil were examined. The heart was fixed in a 10% formalin solution and the coronary arteries were injected with neoprene latex 450®. The myocardial bridge is a superficial muscular band that crosses a short segment of the coronary arteries on the epicardium at various locations in the heart. Twelve bridges were seen in this heart. Three crossed the first branch, five crossed the second branch and four crossed the fifth branch of the paraconal interventricular branch of the left coronary artery. The average length of the myocardial bridges was 2.08 mm (range 0.80-3.95 mm), the length of the left ventricle was 58.3 mm. Of the bridges examined 58.3% were in the medial third of the ventricle, 33.3% were in the apical third and 8.3% were in the basal third.