Journal of Morphological Sciences
http://www.jms.periodikos.com.br/article/587cb4477f8c9d0d058b45a8
Journal of Morphological Sciences
Original Article

EFFECTS OF SOMATOSTATIN, LHRH AND TGF-α ON EPITHELIAL CELL PROLIFERATION IN FETAL STOMACH MAINTAINED IN ORGAN CULTURE

Eliane Maria Goldfeder; Eliana Parisi Alvares

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Abstract

An organ culture technique was used to examine the effects of somatostatin, luteinizing hormone releasing hormone (LHRH) and transforming growth factor (TGF-α) on epithelial cell proliferation in rat fetal stomach. The explants were obtained from 20-day rat fetuses and were maintained in organ culture for 24 h. Half of the culture dishes were supplemented with 10% fetal bovine serum (FBS). Cell proliferation was assessed using the metaphasic index. Light and electron microscopy showed that the explants could be maintained in good condition, independent of the FBS or hormone treatment. Re-epithelialization occurred at the edges of the fragments. The addition of 10% FBS was not advantageous for evaluating cell proliferation in this organ culture system. The low metaphasic index showed that somatostatin and LHRH significantly inhibited cell proliferation after 24 h of treatment. In contrast, TGFα had a mitogenic effect on fetal gastric mucosa and prevented glandular degeneration. These results corroborate our previous studies in vivo and provide direct evidence of the influence of hormonal and growth factors on gastric mucosa during fetal development.

Keywords

Cell proliferation, growth factor, hormones, organ culture, stomach
587cb4477f8c9d0d058b45a8 jms Articles
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J. Morphol. Sci.

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